Bombyx mori ELAV-like-1(BmEL-1) and Bombyx mori ELAV-like-2(BmEL-2) are two members of the ELAV-like family of RNA-binding proteins. Mutations in Bmel-1 and Bmel-2 resulted in5.8% and 28.5% decreases in larval weight on the third day of the fifth instar larva(L5D3), respectively.Triglycerides(TG) are the most important energy resource and also is the main component of neutral fat(NF) in animals. To investigate the role of Bmelav-like genes in the synthesis and decomposition of TG,transcriptomic and metabolic analyses were performed on the whole bodies on the first day of second instar larvae(L2D1) and on fat bodies on L5D3 of Bmel-1-and Bmel-2-mutants, respectively. The diglyceride(DG) and phosphatide(PA) contents were significantly lower in Bmel-1-and Bmel-2-fat bodies than those of the control group. Consistently, the NF contents of both mutants' fat bodies were reduced by 50% and60% respectively. BmEL-2 positively regulates BmAGPATγ and BmFAF2 expression by binding to the specific regions of their 3' UTRs in BmN cells. This study suggests that BmEL-2 is an important regulator of BmAGPATγ and BmFAF2 expression and thereby controls TG metabolism in the silkworm fat body.