Moderate dietary restriction(DR) is widely regarded as the most effective and health-promoting intervention for life extension. However, there remains a lack of conclusive evidence to endorse long-term nutritionally balanced DR as a safe strategy for promoting healthy longevity. In this study, we leveraged the advantages of the silkworm(Bombyx mori) as a model organism in medical research to develop an across-generational animal model of DR. We then analyzed the effects of moderate across-generational DR on various life indicators, key biochemical indices, and genes associated with DR metabolism in the silkworm. The results indicated that long-term DR led to a reduction in body weight in silkworms, which subsequently stabilized at a relatively constant level, and a significant life-extending effect was observed and sustained along with the generation accumulation.Despite a reduction in fertility(spawning amount) and economic traits(cocoon shell rate), the silkworm successfully reproducted generation to generation with adverse effects diminishing as dietary restriction increased. And their biochemical indices revealed a significant enhancement in the antioxidant capacity compared to the libitum feeding(AL) silkworm.Notably, there was a marked difference between groups subjected to successive and contemporary dietary restriction. The expression levels of genes related to oxidative stress were significantly up-regulated or down-regulated, depending on their specific roles. These results suggest that sustained moderate DR can extend lifespan and decrease both weight and fat, primarily due to enhanced antioxidant capacity. Furthermore, our findings indicate that animals exhibit adaptability to prolonged moderate DR in terms of reproductive and generational aspects, thereby demonstrating the feasibility of across-generational moderate DR in insects. Our study not only corroborates that enhancing antioxidant capacity constitutes a health-promoting and life-prolonging strategy for animals subjected to DR, but also preliminarily illustrates the adaptive capacity of animals to DR across multiple generations.These findings provide a foundational basis for the development of long-term, moderate,nutritionally balanced, and safe dietary programs for humans and large animals.