Baculovirus is a promising biopesticide, but the relatively narrow host domain of baculovirus will limit its application as a biocide. Autographacalifornica nucleopolyhedrovirus(AcMNPV)is a wide host baculovirus, while it barely infects Bombyx mori(B. mori, Lepidoptera). Previously, we found that AcMNPV can infect p50 silkworm through puncture, and B. mori eukaryotic Initiation factor4E-2(BmeIF4E2) was identified to be might the key gene in AcMNPV infection. Studying its function has great significance in enlarging baculovirus host domain. In order to preliminarily predict the function of BmeIF4E2, the sequence characteristics and evolutionary relationship analysis showed that BmeIF4E2 was relatively conservative. In addition, the Spatiotemporal Expression Pattern of BmeIF4E2 was found highly expressed in the egg, during molting of the 4th, and pupal stage. In order to further explore the function of BmeIF4E2, AcMNPV was detected through overexpression and Knockdown BmeIF4E2. It shows that AcMNPV was consistent with the expression level of BmeIF4E2.To verify the relationship between AcMNPV and BmeIF4E2, His-tag pull down assay shows that BmeIF4E2 can interact directly with BV-eGFP.