Silkworm disease presents a significant obstacle to the progress of the sericulture industry. Analyzing the disease resistance mechanism of the domestic silkworm is an important research focus for disease prevention and control. It is also a core issue in understanding the interaction between pathogens and hosts.There is an urgent need to thoroughly investigate the resistance mechanisms of domestic silkworms and overcome the limitations imposed by silkworm disease on industry development. The BmNPV disease of silkworms is the main disease affecting the sericulture industry, causing significant economic losses to China's sericulture industry each year. Effective explanations for the mechanisms of resistance to BmNPV and the key genes for antiviral defense in domestic silkworms are still lacking. Consequently, there is a need to systematically elucidate the resistance mechanisms and provide theoretical support and fundamental data for breeding resistant material. This study focuses on analyzing the infection characteristics and differences of BmNPV in the 871C and 871 domestic silkworm variety. It isolates the progeny viruses produced by BmNPV infection in 871C and 871 and analyzes their virus structure characteristics. The study also utilizes proteomics to identify different proteins in the progeny virus particles produced by different variety. Furthermore, it screens and identifies key host proteins through protein interaction and molecular biology methods, elucidating the molecular mechanism for the production of defective virus particles by the resistant variety. This research project aims to elucidate the antiviral mechanism of the resistant silkworm strain 871C and provide a theoretical basis for breeding disease-resistant domestic silkworms.